Effects of carbon dioxide pneumoperitoneum on the invasive ability of ovarian carcinoma cell line SKOV3
- VernacularTitle:二氧化碳气腹环境对人卵巢癌细胞系SKOV_3侵袭能力的影响
- Author:
Junying TANG
;
Jin HUANG
;
Zhenwei YAO
- Publication Type:Journal Article
- Keywords:
carbon dioxide pneumoperitoneum;
ovarian tumor;
adhesiveness;
tumor invasion
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanism of carbon dioxide (CO_ 2 ) pneumoperitoneum on the adhesive, migratory and invasive abilities of ovarian carcinoma cell line SKOV_ 3 . Methods SKOV_ 3 cells were exposed to an CO_ 2 insufflation medium. The cells′ adhesive ability to Matrigel was analyzed by MTT assayand, and their migratory and invasive abilities were analyzed through Boyden filters metastasis model. CD44 and bFGF expressions were examined by immunohistochemistry and semi-quantitative RT-PCR. Results Cells exposed to an in vitro pneumoperitoneum demonstrate could significantly increase adhesive, migratory and invasive abilities compared to the control group (P
