Curcumin inhibits the activation marker of hepatic stellate cells by up-regulating the peroxisome proliferator-activated receptor?.
- VernacularTitle:姜黄素上调PPAR?抑制肝星状细胞活化标志表达的研究
- Author:
Yang CHENG
;
Jian PING
;
Lieming XU
- Publication Type:Journal Article
- Keywords:
Hepatic stellate cell;
Curcumin;
Peroxisome proliferator-activated receptor?;
?Smooth muscle actin
- From:
Chinese Journal of Practical Internal Medicine
2006;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relation between the effect of Curcumin on the proliferation and activation of hepatic stellate cells(HSC)and the expression of the peroxisome proliferator-activated receptor?(PPAR?).Methods The rat HSC was isolated from SD rats through in situ perfusion of liver with Pronase E and density-gradient centrifugation with Nycodenz.The subcultured cells were treated with corresponding compound.The inhibition effect on HSC proliferation was determined by MTT colorimetry.The total RNA was extracted by TRizol reagent,and the gene expression level of PPAR? and smooth muscle actin (?-SMA)were determined by semi-quantitative RT-PCR.The total cellular proteins were extracted and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis,and the protein level of ?-SMA was determined by Western blotting.Results The MTT analysis results showed that Curcumin inhibited HSC proliferation between 10 and 50 ?mol/L in a dose-dependent manner.In the Day 1,4,7 of primary HSC and passaged HSC,the expression of PPAR? mRNA decreased with HSC activation extent;Curcumin could up-regulate the expression level of PPAR?.Curcumin suppressed the expression of ?-SMA not only at the gene level but also at the translation level.But all these effects of Curcumin on HSC could be blocked by the PPAR? specific antagonist GW9662.Conclusion The effect of Curcumin on HSC cell proliferation and activation is through the up-regulation of PPAR? signal.
