Probiotics in the treatment of experimental colitis in rats and its influence on T cell subsets.
- VernacularTitle:益生菌治疗大鼠实验性结肠炎效果观察及对T细胞亚群的影响
- Author:
Yulan LIU
;
Ning CHEN
;
Peng YOU
- Publication Type:Journal Article
- Keywords:
Probiotics;
Trinitrobenzenesulphonic acid;
Experimental colitis;
T cell subset
- From:
Chinese Journal of Practical Internal Medicine
2006;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of probiotics in experimental colitis of rats.Methods Models of experimental colitis were established.Several groups of rats were set up as probiotics group,negative group and positive group.Histologic scoring was used to estimate effects of drugs;the expression of CD~+_4 andCD~+_8 on T cell surface in peripheral blood,spleen mononuclear cells and intraepithelial lymphocytes of colon were detected by flow cytometry.Results Histopathology shows that probiotics of larger dosage was curative for experimental colitis in rats.Proportion of CD~+_4 and CD~+_8 T cells increased in colon of colitis,which was decreased by treatment of larger dose of probiotics.CD~+_4/CD~+_8 ratio and T cell subsets in systemic immune system were not influenced by probiotics.Conclusion Probiotics of large dosage are effective in the treatment of experimental colitis of rats incuced by TNBS,possibly associated with immune regulation and tends to be localized in mucosal immune system in colon.
