Inhibition of Bcl-2 Antisense Oligonucleotide Combined with Radiation against Lung Carcinoma Cells
- VernacularTitle:Bcl-2反义寡核苷酸联合照射对肺癌细胞的抑制作用
- Author:
Wei HU
;
Guoping SHAN
;
Changhui YU
- Publication Type:Journal Article
- Keywords:
lung cancer;
Bcl-2;
antisense oligonucleotide;
radiation
- From:
Journal of Zhejiang Chinese Medical University
2007;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To study the inhibitory effect of bcl-2 antisense oligonucleotide(ASODN)combined with radiation on lung carcinoma cells NCI-H446.[Method]The cultured NCI-H446 lung carcinoma cells were divided into 5 groups:control,pure radiation,nonsense+radiation,lipofectin+radiation,ASODN+radiation.Every group except for control were assayed by MTT on the inhibition rates,24h,48h,72h after 10Gy irradiation respectively.[Results]The inhibitions of lung carcinoma cells NCI-H446 were observed in ASODN+radiation,lipofectin+radiation,nonsense+radiation and pure radiation groups.The differences between the ASODN+radiation group and the other three groups were significant(P
