Enzyme-histochemical observation on influence of suspension concentrate of niclosamide in Oncomelania hupensis snails
- VernacularTitle:氯硝柳胺悬浮剂对钉螺影响的酶组织化学观察
- Author:
Hongjun LI
;
Yousheng LIANG
;
Jianrong DAI
;
Ming XU
;
Weiwei RU
;
Yongliang XU
- Publication Type:Journal Article
- Keywords:
25% suspension concentrate of niclosamide;
Oncomelania hupensis snails;
Enzyme;
Enzyme-histochemistry
- From:
Chinese Journal of Schistosomiasis Control
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of suspension concentrate of niclosamide on the enzyme activity of Oncomelania hupensis in order to explore its molluscicidal mechanism. Methods Oncomelania hupensis snails were collected in the habitates of river marshland in Dantu District, Zhenjiang City, Jiangsu Provence and were divided into 2 groups. The snails of the treated group were sprinkled with 25% suspension concentrate of niclosamide. The snails of the control group were sprinkled with distilled water. The soft body tissue of the snail was separated and the sections of snail tissue were made in the Cryostat Microtome. The stain of enzyme-histochemistry showed CCO, LDH, SDH, CHE and NOS had been done, and then the staining block was made by routine method. The staining reaction in the snail tissue and the average gray density were observed with the image analysis system of biomicroscope. Results The enzyme activity of CCO, LDH, SDH, CHE and NOS located in the mouth, muscle fiber, tegumentary membrane, ganglia, liver and pharyngeal cavity of Oncomelania hupensis snails. The enzyme activities of CCO, LDH, SDH, CHE and NOS in the treated group were significantly lower than that in the control group. Conclusions Niclosamide can affect the transmitting of neurohypophysis and obstruct the energy and result in the disorder of the physiological functions in Oncomelania hupensis. It is one of the reasons of Oncomelania hupensis death.
