Effects of smoking on nicotinic acetylcholine receptor subunits expression in peripheral blood mononuclear cells
- VernacularTitle:吸烟对外周血单个核细胞表达烟碱乙酰胆碱受体的影响
- Author:
Bin XIONG
;
Yuerong ZHU
;
Hong QIU
- Publication Type:Journal Article
- Keywords:
smoking;
nicotine;
peripheral blood mononuclear cells;
receptor,nicotinic
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of nicotinic acetylcholine receptor (nAchR) subunits, and the effects of smoking on its expression in peripheral blood mononuclear cells (PBMCs). Methods Blood was collected from 92 healthy male smokers and 36 healthy male non-smokers. Peripheral blood monocytic cells were obtained, and reverse transcription-polymerase chain reaction (RT-PCR) was employed to study the gene expression of 11 nAchR subunits (?2-?7, ?9, ?10, ?2-?4). Results The results showed that the PBMCs of smokers and non-smokers appeared to express ?2, ?5, ?6 and ?7 only out of 11 nAchR subunits. However, the expression of ?5 and ?7 subunits was lower in smokers′ PBMCs than in non-smokers′ (P
