The effect of overexpression of sphingosine kinase on biological characters of gastric carcinoma cells
- VernacularTitle:高表达鞘氨醇激酶对胃癌细胞生物学特性的影响
- Author:
Qiang GUO
;
Rong LI
;
Qingfang LI
- Publication Type:Journal Article
- Keywords:
sphingosine kinase;
gastric carcinoma cell;
apoptosis;
migration
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of sphingosine kinase (SPK) on the regulation of proliferation, migration and apoptosis of gastric carcinoma cells. Methods BGC-823 cells were infected with adenoviral carriers bringing the wild type SPK gene and SPK mutant (SPK DN ), and their effect on the migration and 5-FU-induced apoptosis of BGC-823 cells was evaluated. The adenovirus-mediated expression of SPK was detected by Western blot, and cellular SPK enzyme activity was assayed. The cell migration was investigated by Transwell Migration Technology. Results The adenovirus-mediated wild type SPK gene increased the cellular SPK activity, thus leading to enhanced migration and resistance to 5-FU-induced apoptosis of BGC-823 cells. Whereas overexpression of SPK mutant (SPK DN ) decreased the cellular SPK activity, thus inhibited the migration and sensitized BGC-823 to 5-FU-induced apoptosis. Conclusion SPK plays important roles in the regulation of migration and apoptosis of gastric carcinoma cells, therefore it may be of a new target for tumor treatment.
