Primary culture and purification of embryonic dorsal root ganglion neurons
- VernacularTitle:胚胎大鼠背根神经节神经元的培养与纯化
- Author:
Jun ZHANG
;
Bainan XU
;
Chong LI
- Publication Type:Journal Article
- Keywords:
cell culture;
ganglia, spinal;
neurons
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and reliable method for primary culture and purification of embryonic dorsal root ganglion neurons. Methods Dorsal root ganglions (DRG) from rat embryos were isolated under microscope and dissociated in trypsin. The dissociated neurons were treated with 5-fluorouracil for purification. Immunocytochemistry was performed by double staining with anti-calcitonin gene-related peptide and anti-NF200 antibodies to evaluate the purification rate of DRG neurons. Results Isolated and cultured DRG neurons survived and grew nicely in the basis medium for neurons, and the purified rate of DRG neurons could reach to around 93%. Conclusion The above method is very useful to obtain dorsal root ganglion neurons and to purify the neuronal population.
