Effects of induction with low concentration of ethanol and ultraviolet irradiation on cell cycle and the expression of p53 and p21 in HepG2 cell line
- VernacularTitle:低浓度乙醇诱导与紫外线照射对HepG2细胞周期及p53/p21蛋白表达的影响
- Author:
Xiuli ZHANG
;
Jianhui QU
;
Ziqin SUN
- Publication Type:Journal Article
- Keywords:
ethanol;
ultraviolet rays;
cell cycle;
protein expression
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the possible molecular mechanism of the damage of HepG2 cell line induced by low concentration of ethanol and ultraviolet irradiation. Methods Hepatoma cell line (HepG2) was exposed to low concentration of ethanol and ultraviolet irradiation, then the changes in cell cycle, the expression of p53 and p21, the correlation between expression of p53 and p21 and cell cycle were analyzed. Results The expression of p53 and p21 was greatly increased, and the cell cycle was found to be blocked at G_0-G_1/G_2-M after ultraviolet irradiation. The cell cycle block of G_2-M and the increased expression of p21 were observed when exposed to low concentration of ethanol, while the expression of p53 showed no significant changes. Cell apoptosis was increased when the cells were exposed to the both injury factors. Conclusion Low concentration of ethanol and ultraviolet irradiation can affect cell cycle and the process of cell apoptosis through activation of different molecular mechanism.
