Quantitative determination of Fas ligand and T-cell intracellular antigen-1 in renal allografts
- VernacularTitle:移植肾组织Fas配体和T细胞内抗原-1表达水平的定量分析
- Author:
Wanjun ZHANG
;
Changlin MEI
;
Chaoyang YE
- Publication Type:Journal Article
- Keywords:
kidney transplantation;
graft rejection;
FasL/TIA-1
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the quantitative method of determination of Fas ligand (FasL) and T-cell intracellular antigen-1 (TIA-1) in renal allografts and to evaluate its clinical significance. Methods The magnitude of intragraft FasL and TIA-1 mRNA was quantified by competitive PCR in 42 samples obtained to diagnose the etiology of graft dysfunction, and the results were expressed as the ratio of picograms of target gene to picograms of ?-actin, and were compared with the histopathogical diagnosis based on Banff criteria. Results Of all 42 samples, the transcripts were detectable in 11 for FasL and 27 for TIA-1. Intragraft expression of FasL and TIA-1 was significantly up-regulated in acute rejection group compared with chronic rejection group (P
