Dynamic changes of calcium ions in retinal ganglion cells exposed to hypoxia and their relationship with TNF-? and lipid peroxidation
- VernacularTitle:RGCs缺氧条件下钙离子水平动态变化及与TNF-?及脂质过氧化反应的关系研究
- Author:
Peng CHEN
;
Xiangge HE
- Publication Type:Journal Article
- Keywords:
RGCs;
calcium ions;
SOD;
MDA;
TNF-?
- From:Journal of Third Military Medical University
2003;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of retinal ganglion cells (RGCs) exposed to hypoxia and the mechanism. Methods RGCs were isolated from the retina of neonatal Long Evans rats aged 1 day and cultured, then divided into normal control group and hypoxia group (cultured in incubator containing 1 O_2, 5 CO_2 and 94 N_2). At 1, 3, 12 and 24 h after incubation, the calcium ion level by laser scanning confocal fluorescence microimaging system, the activity of SOD and the content of MDA by biochemistry technology, TNF-? by ELISA were detected. Results No changes of calcium ion level in RGCs were observed in normal control group. The calcium ion level increased significantly in the hypoxia group (P
