Distribution and significance of NGF in cochleae of neonate rats
- VernacularTitle:神经生长因子在新生大鼠耳蜗的定位及其意义
- Author:
Jun CHI
;
Xueyuan ZHANG
;
Wuzhan SONG
- Publication Type:Journal Article
- Keywords:
NGF;
cochlea;
immunohistochemistry
- From:Journal of Third Military Medical University
2003;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of NGF in the cochleae of neonate rats and to explore the mechanism of NGF in the auditory path. Methods Twenty healthy neonate SD rats aged 5-7 d were selected and their cochlear paraffin sections were labeled with a rabbit anti-NGF polyclonal antibody by SP method. Results The membranous labyrinth of neonate rats appeared similar to triangle and NGF expression was observed in immature corti’s organ, in spiral ganglion cells and in nerve fibers throughout the cochlea. The distribution located in both cytoplasm and nucleus. Conclusion The distribution of NGF was closely related to survival and maturation of spiral ganglion cells and hair cells in rats aged 5-7 d.
