Effect of resveratrol on peripheral blood mononuclear cells oxidative DNA damage in type 2 diabetes mellitus patients in vitro
- VernacularTitle:白藜芦醇对2型糖尿病患者外周血单个核细胞DNA损伤的体外修复
- Author:
Zhong JIANG
;
Xulei TANG
- Publication Type:Journal Article
- Keywords:
type 2 diabetes mellitus;
oxidative stress;
DNA damage;
resveratrol
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the oxidant-antioxidant status and levels of mononuclear cells (PBMCs) oxdative DNA damage in patients with type 2 diabetes mellitus (T2DM), and to value the effect of resveratrol on peripheral blood mononuclear cells oxidative DNA damage in T2DM patients in vitro. METHODS: The DNA damages in PBMCs of 39 T2DM patients with vascular complication, and 29 T2DM patients without complication were detected by single cell gel electrophoresis (the comet percentage). The levels of plasma total antioxidative capacity (TAC), the contents of plasma malondialdehyde (MDA) and plasma thiol group (SH) were measured. The effect of resveratrol on DNA damage of T2DM patients were investigated in vitro. In addition, 26 healthy subjects were studied as a control group. RESULTS: The comet percentage of PBMCs and the contents of plasma MDA in T2DM patients without complication were all significantly higher than that in healthy controls (P
