Expression of matrix metalloproteinases-1/tissue inhibitor of metalloproteinase-1 in kidney of patients with autosomal dominant polycystic kidney disease
- VernacularTitle:基质金属蛋白酶1/组织金属蛋白酶抑制因子1在常染色体显性遗传性多囊肾组织中的表达
- Author:
Xingang CUI
- Publication Type:Journal Article
- Keywords:
matrix metalloproteinases-1;
tissue inhibitor of metalloproteinase-1;
kidney;
kidney transplantation;
polycystic kidney,autosomal dominant
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the differential expression of matrix metalloproteinases-1 / tissue inhibitor of metalloproteinase-1 (MMP-1/TIMP-1) between normal kidney,kidneys of patients with autosomal dominant polycystic kidney disease(ADPKD),and the original kidneys after renal transplantation(OKRT).Methods: DNA microarray technique was used to analyze the differential gene expression in the above 3 tissues.Semi-quantitive RT-PCR was performed to verify the differentially expressed genes.Results: There were 463 differentially expressed genes between normal kidney and ADPKD tissues and 130 differentially expressed genes between ADPKD and the OKRT tissues.Expression of MMP1/TIMP1 in the ADPKD and the OKRT tissues were significantly higher than that in the normal kidney tissue(P
