Influence of ischemia/reperfusion on characteristics of membrane potassium channels of human renal tubule epithelial cells
- VernacularTitle:缺血再灌注对人肾小管上皮细胞钾离子通道特性的影响
- Author:
Hao CHEN
- Publication Type:Journal Article
- Keywords:
patch-clamp techniques;
potassium channel;
anoxia;
kidney tubulars;
epithelial cells
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the influence of ischemia/reperfusion(simulated by anoxia) on the characteristics of K~(+) channels in renal tubular epithelial cells.Methods: Healthy renal tubule epithelial cells were divided into normal control group(cultured in serum-free RPMI 1640 medium),anoxic group(treated with antimycin A + serum-free RPMI 1640 medium),and oxidative stress group(H_(2)O_(2)+ serum-free RPMI 1640 medium)(n=15).The whole-cell patch clamp technique was used to examine the characteristics of K~(+) channels in renal tubular epithelial cells.Results: The transmembrane K~(+) current in tubular epithelial cells was voltage-dependent and could be blocked by K~(+) channel blocker Ba~(2+).The transmembrane potential levels in anoxic group and oxidative stress group were remarkably higher than that in normal control group(P
