Establishment of a ?B2 crystallin gene knockout mice model
- VernacularTitle:?B2晶体蛋白基因敲除小鼠模型的建立
- Author:
Jun ZHANG
- Publication Type:Journal Article
- Keywords:
beta-crystallin;
mice,gene knockout
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a ?B2 crystallin gene knockout mice model.Methods: The gene-targeting vector was established by the technique of American inGenious Targeting Laboratory.PCR technique was used to identify the genotype of the model mice and expression of ?B2 crystallin protein was detected by Western blot.Results: Three genotypes were successfully(identified) and expression of ?B2 crystallin protein was not detected in gene knockout mice.Conclusion: The ?B2 crystallin gene knockout mice model has been successfully established.
