Antagonist of injection salvia miltiorrhiza on streptomycin ototoxicity of guinea pigs
- VernacularTitle:丹参注射液拮抗链霉素耳中毒的实验研究
- Author:
Lijuan SHI
;
Hao TANG
;
Yuxiang AN
- Publication Type:Journal Article
- Keywords:
injection salvia miltiorrhiza;
streptomycin(SM);
cochlea;
apoptosis;
basic fibroblast growth factor(bFGF)
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To examine the apoptosis and bFGF expression on the cochlea of guinea pig after administrated streptomycin(SM) and the antagonism of Salvia Miltiorrhiza Injection(DS) on SM ototoxity.Methods Forty guinea pigs were divided into four groups,and treated with DS and SM respectively.The apoptosis and bFGF expression were examined by TdT mediated biotin dUTP nickend labeling(TUNEL) techniques and SABC immunohistochemical staining with paraffin slide.The intensity of images were stored in a computer and analyzed by the image quantitative analysis technique.Auditory brainstem response(ABR) measurement was used to detect the ototoxicity before/after the examination.Results After 10 d treated by drugs,the ABR threshold of SM increased significantly,while the DS+SM reduced more(P
