Expression of growth-associated protein in hippocampus of neonatal rats with hypoxic-ischemic brain damage
- VernacularTitle:生长相关蛋白在缺氧缺血性脑损伤新生大鼠海马中的表达变化
- Author:
Liqun LU
;
Congmin ZHAO
;
Zhaoxia PU
- Publication Type:Journal Article
- Keywords:
cerebral hypoxia;
cerebral ischemia;
growth-associated protein;
hippocampus;
rat
- From:Journal of Third Military Medical University
2003;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of growth-associated protein-43(GAP-43) in the hippocampus of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods HIBD was established by the method of Rice in 48 SD rats aged 7 days and another 48 matched normal rats served as control. Immunohistochemistry and RT-PCR were respectively used to detect the expression of GAP-43 protein and mRNA in all rat hippocampus on day 8, 10, 14, 21, 28, 35 after HIBD (n=8 at each time point for each group). Results The expression of GAP-43 protein and mRNA in hippocampus was obviously increased in HIBD rats as compared with normal rats, the peak of GAP-43 protein appeared at 3rd week, and the peak of mRNA at 2nd week. Conclusion The expression of GAP-43 protein and mRNA increased in the hippocampus of rats following HIBD, which was possibly related to the recovery of injured hippocampus.
