Low salt stimulates the expression of cyclooxygenase-2 and its relationship to p38 MAPK signal transduction in a mouse macula densa cell line
- VernacularTitle:低盐诱导的致密斑细胞环氧化酶2表达与p38丝裂素激活蛋白激酶信号通路
- Author:
Dongyan LIU
;
Xuewang LI
;
Hang LI
;
Yubing WEN
;
Lin DUAN
;
Yan LI
- Publication Type:Journal Article
- Keywords:
Cyclooxygenase 2;
Prostaglandins E;
p38 Mitogen-activated protein kinases;
MAP kinase signaling system;
Saline solution, hypotonic;
Macular densa cells
- From:
Chinese Journal of Nephrology
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of low salt (LS) medium and the involvement of p38 mitogen-activated protein kinase (p38 MAPK) in the expression of cyclooxygenase-2 (COX-2) and production of PGE2 in a mouse macula densa derived cell line (MMDD1 cells). Methods Confluent MMDD1 cells were exposed to serum free DMEM/F12 in a 1:1 mixture with normal saline (NS), 300 mmol/L mannitol (to reduce NaCl to half, LS solution). Semi-quantitive reverse transcription and polymerase chain reaction amplication (RT-PCR) and Western blotting were used to detect the expression of COX-2 mRNA and protein with LS solution. The content of PGE2 in the supernatant was examined by enzyme linked immunosorbent assay (ELISA). Expression of total and phosphorylated p38 MAPK kinsae was examined by Western blotting in LS solution. Results The COX-2 mRNA abundance and protein expression of MMDD1 cells were up-regulated in LS group as compared to NS group (16 h mRNA 0.94?0.12 vs 0.26?0.09, 28 h protein 0.59?0.02 vs 0.25?0.07, rspectively, all P
