Inhibitory effects of ?-lipoic acid on activation of NF-?B induced by high glucose in rat mesenteric cells
- VernacularTitle:?-硫辛酸对高糖诱导的系膜细胞核因子-?B活性的抑制作用
- Author:
Hui LI
;
Yitao JIA
;
Duo WEI
- Publication Type:Journal Article
- Keywords:
lipoic acid;
NF-kappa B;
mesangial cells
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ?-lipoic acid on the proliferation and activation of NF-?B induced by high glucose (HG) in rat mesenteric cells (MCs). Methods The rat mesenteric cells were cultured in the medium with normal glucose (5.6mmol/L, NG), high glucose (25mmol/L, HG), HG+100 ?mol/L ?-lipoic acid, or HG+200?mol/L ?-lipoic acid and HG+PDTC (a NF-?B inhibitor). Activation of nuclear factor-?B (NF-?B) of rat mesenteric cells were measured by electrophoretic mobility shift assay (EMSA). The cell proliferation was assessed by MTT. Results ?-lipoic acid (50~300?mol/L) can inhibit the proliferation of MCs. The NF-?B binding activity was 2.2 -fold higher in MCs exposed to HG compared to NG (P
