Interference effects of tetramethylpyrazine on the proliferation of vascular smooth muscle cells(VSMCs) induced by angiotensin Ⅱ
- VernacularTitle:川芎嗪对Ang Ⅱ诱导的大鼠VSMCs增殖的干预作用
- Author:
Honghua ZHENG
;
Desheng LUO
;
Yinghong LI
- Publication Type:Journal Article
- Keywords:
tetramethylpyrazine;
cells, muscle, smooth, vascular;
calcineurin;
genes, fos;
proliferating cell nuclear antigen
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the interference effects of tetramethylpyrazine (TMP) on calcinuerin (CaN), c-fos and the nuclear antigen of proliferating cells in the proliferation of vascular smooth muscle cells (VSMCs) treated by angiotensinⅡ(Ang Ⅱ). Methods A cell proliferating model of VSMCs induced by Ang Ⅱ was established; the effects of TMP on CaN was detected by enzyme reaction phosphorus measurement; the effects of TMP on c-fos gene and PCNA expression were observed by immunocytochemical staining and image analysis technique (A value). Results The rats’ aortic smooth muscle cells were cultured in vitro successfully. CaN activities, cell proliferation activity and the expression levels of c-fos and PCNA increased significantly in VSMCs proliferation induced by Ang Ⅱ (P
