Study of oxidative DNA damage in lymphocyte of MHD patients
- VernacularTitle:维持性血液透析患者淋巴细胞DNA氧化损伤的研究
- Author:
Qin ZHAO
;
Haidong YAN
;
Xuezhu LI
- Publication Type:Journal Article
- Keywords:
Maintenance hemodialysis;
Dialysis membrane;
DNA;
Oxidative damage
- From:
Chinese Journal of Practical Internal Medicine
2006;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the oxidative DNA damage in lymphocyte of MHD patients and the effect of dialysis membrane on it through comet assay.Methods From 2003-05 to 2004-10 we selected 20 CRF patients undergoing maintenance hemodialysis and divided the patients into HE and PS group randomly.And 7 volunteers were chosen as controls.The patients in each group had undergone hemodialysis for 12 weeks using HE or PS membrane respectively.Then we took venous blood sample and separated lymphocyte.The oxidative DNA damage was measured by comet assay.Results The comet tail length of control group,HE group and PS group was(0.286?0.194)?m,(0.970?0.120)?m and(0.656?0.187)?m respectively.Compared with control group,the comet tail length of MHD patients was significantly longer(P
