Analysis of cytoplasmic CD_( 79a)expression on acute leukemia cells
- VernacularTitle:细胞分化抗原胞浆CD_(79a)在急性白血病细胞上的表达
- Author:
Jingyu ZHANG
;
Lin YANG
;
Ling PAN
- Publication Type:Journal Article
- Keywords:
Acute leukemia;
CyCD_ 79a;
Immunophenotyping;
Flow cytometry
- From:
Chinese Journal of Practical Internal Medicine
2006;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expression of cytoplasmic CD_ 79a(CyCD_ 79a)on different acute leukemia(AL)cells and to explore its significance.Methods CyCD_ 79aexpression on the leukemic cells from 221 acute leukemia patients diagnosed from January 2003 to April 2004 was retrospectively analyzed by three-color cytometry with CD_ 45/SSC gating strategy.Results CyCD_ 79awas positive in 100%(57/57)of B cell-acute lymphoblastic leukemia(B-ALL)cases,which was higher than that of other B cell-associated antigens(CD_ 10,CD_ 19,CD_ 20and cytoplasmic CD_ 22)in B-ALL.Only 0.7%(1/134)of acute myeloid leukemia(AML)cases and 13.3%(2/15)of T cell-acute leukemia(T-ALL)cases expressed CyCD_ 79a,respectively.Conclusion CyCD_ 79ais sensitive and specific for B-ALL.CyCD_ 79amay be used as a reliable immunophenotyping marker for the diagnosis of B-ALL and be used to distinguish B-ALL from T-ALL and AML.
