Study on variations of mtDNA from high and low metastatic mouse hepatocarcinoma cell sublines
- VernacularTitle:高低转移小鼠肝癌细胞线粒体DNA遗传变异的研究
- Author:
Jigang DAI
;
Yingbin XIAO
;
Jiaxin MIN
;
Guoqiang ZHANG
;
Mingzhang XIANG
;
Qiuping WU
;
Ke YAO
;
Renjie ZHOU
;
Zubin YU
- Publication Type:Journal Article
- Keywords:
mitochondrial DNA;
hepatocarcinoma;
tumor;
variation
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the variations of mtDNA from high and low metastatic mouse hepatocarcinoma cell sublines Hca-F and Hca-P, and the relationship between mutations of mtDNA and carcinogenesis. Methods The variations of D-loop, ND3 and tRNA Met+Glu+Ile gene fragments of mtDNA from Hca-F and Hca-P cells were analyzed by PCR-RFLP and sequencing techniques. Results No amplification fragment length polymorphism and restriction fragment length polymorphism were observed in tRNA Met+Glu+Ile , ND3 and D-loop of mtDNA from the 2 cell sublines. Sequence difference between these 2 cell sublines were found in mtDNA D-loop region by sequencing. Conclusions Genetic alteration of mtDNA non-coding region in tumors, which may reflect the environmental and genetic influences operative during tumor progression, can be linked to their tumorigenic phenotype.
