Effect of matrilysin and its antisense oligonucleotide on proliferation of lung adenocarcinoma cell line A549
- VernacularTitle:基质溶解素及其反义寡核苷酸对肺腺癌A549细胞增殖的作用
- Author:
Zhixin WANG
;
Fei XU
;
Qichao XIE
;
Zhengtang CHEN
- Publication Type:Journal Article
- Keywords:
matrilysin, MMP-7-antisense oligonucleotide, human umbilical vein endothelial cell, lung adenocarcinoma cell line A549
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of the recombinant matrilysin (rMMP-7) and its antisense oligonucleotide transfected by liposome (LIPO-MAON) on the proliferation of lung adenocarcinoma cell line A549 and human umbilical vein endothelial cells (HUVEC). Methods Antisense oligonucleotide of matrilysin was constructed by liposome transfection. The proliferation of HUVEC and A549 was detected by MTT assay in case of rMMP-7 or LIPO-MAON existence. The expression of MMP-7 mRNA in both HUVEC and A549 was examined by semi-quantitative RT-PCR assay. Results The proliferation of both HUVEC and A549 cell line was accelerated by high level of rMMP-7 (0.75, 1.0 ?g/ml), and the inhibited proliferation was only found in A549 cell line treated with high concentration of LIPO-MAON (7.5, 10 nmol/ml), but not in HUVEC. By RT-PCR assay, no expression of MMP-7 mRNA was found in HUVEC; however, enhanced or decreased expression of mRNA were found when A549 cell line was treated respectively with rMMP-7 or LIPO-MAON (P
