Effects of early enriched environment intervention on GAP-43 expression in rat hippocampus after hypoxic-ischemic brain injury
- VernacularTitle:早期丰富环境干预对缺氧缺血性脑损伤新生鼠海马GAP-43表达的影响
- Author:
Liqun LU
;
Congmin ZHAO
;
Zhaoxia PU
- Publication Type:Journal Article
- Keywords:
cerebral hypoxia;
cerebral ischemia;
early intervention;
environment;
growth-associated protein
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of early enriched environment intervention on the expression of growth-associated protein-43(GAP-43) in the hippocampus of rats with hypoxic-ischemic brain injury (HIBI). Methods After the establishment of HIBI model in SD rats by the method of Rice, the animals were divided randomly into 2 groups: the intervention group and non-intervention group. The sham-operation rats were used as control group. Enriched environment intervention had been administrated to the rats of intervention group since the 2nd day after HIBI. On the 3rd, 7th, 14th, 21st and 28th day, immunohistochemistry and RT-PCR were used to measure the expressions of GAP-43 protein and mRNA in the hippocampus of rats. Results The expression of GAP-43 protein and mRNA in the rat hippocampus were increased in the non-intervention group than in the control group(P
