Therapeutic effects of basic fibroblast growth factor on morphological features of hippocampal neurons in hyperbilirubinemia rats
- VernacularTitle:碱性成纤维细胞生长因子对高胆红素血症模型鼠海马神经元形态改变的影响
- Author:
Yu WANG
;
Yuxi ZHU
;
Xiangyin KONG
- Publication Type:Journal Article
- Keywords:
hyperbilirubinemia;
GAP-43;
electromicroscopy;
bFGF
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the morphological damages of hyperbilirubinemia on rat central nervous system, and evaluate the therapeutic effects of different routes of basic fibroblast growth factor (bFGF) administration. Methods Totally 90 Wistar rats were inflicted to hyperbilirubinemia models and then divided randomly into 3 groups:untreated group (BG),treated-1 group (T_ 1 G), treated-2 group (T_ 2 G), and another 30 rats served as control group (CG). bFGF injection were given at 30 min, 1, 3, 5 and 7 d by abdominal cavity (T_ 1 G,) and cranial cavity (T_ 2 G). GAP-43 expression levels and the micro-morphological features were observed with immunohistochemical assay and transmission electron microscopy respectively at 2 nd, 8 st, 28 st and 60 st day. Results ①Hyperbilirubinemia rat model was verified by gross and microscopic observation, bilirubin concentrations in the plasma and brain.②Compared to CG, the expression level of GAP-43 was significantly up-regulated in BG, T_ 1 G and T_ 2 G, especially in the later 2 groups (P
