Immunohistochemical analysis of neuropeptide Y in the Bullfrog Retina
- VernacularTitle:牛蛙视网膜内神经肽Y的免疫组织化学研究
- Author:
Xia ZHAO
- Publication Type:Journal Article
- Keywords:
retina;
neuropeptide Y;
amacrine cells;
synaptic connectivity;
post-embedding colloidal gold labeling electron microscopy
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the distribution and synaptic connectivity of neuropeptide Y (NPY)-like immunoreactivity (IR) in Bullfrog retina, Rana Calesbeiana, especially in the inner plexiform layer. Methods Light microscopy immunofluorescence and post-embedding colloidal gold labeling electron microscopy techniques were employed in this study. Results NPY immunoreactivity was associated with a small population of amacrine cell bodies in the inner nuclear layer and cell processes, ramified with 3 distinct bands, in the inner plexiform layer. As for ultrastructure, quantitative analysis showed that NPY-IR amacrine cell processes were most often presynaptic to NPY negative amacrine cell processes (49.7%) and ganglion cell dendrites (49.3%). NPY-IR amacrine cells predominantly received synaptic input from bipolar cell axon terminals (86%), while a few input from other amacrine cell processes (14%). Conclusion NPY-IR in Bullfrog retina is mainly distributed in the inner part and predominately receives excitatory glutamate modulation from bipolar cells.
