EFFECTS OF GRAPE JUICE ON PROLIFERATION AND APOPTOSIS OF HUMAN PROSTATE CARCINOMA PC-3 CELLS
- VernacularTitle:葡萄汁对人前列腺癌PC-3细胞增殖和凋亡的影响
- Author:
Qiong LUO
;
Yaqin PANG
;
Mingliang YANG
;
Jun YAN
;
Lihong WANG
;
Xiaoyan CUI
- Publication Type:Journal Article
- Keywords:
grape juice;
resveratrol;
human prostate carcinoma PC-3 cells
- From:
Acta Nutrimenta Sinica
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To study the inhibitory effects of grape juice on human prostate carcinoma PC-3 cells and its possible mechanism. Method: PC-3 cells were treated with grape juice in different concentration(16, 32, 64 ?l/ml) and resveratrol for 48 h; and the proliferation of PC -3 cells were measured by growth curve and MTT assay. TUNEL was used to observe the morphology of apoptotic cells and flow cytometry to analyze the PC-3 apoptosis. Results: Resveratrol and grape juice could markedly inhibit the proliferation of PC-3 cells, and TUNEL positive cells were detectable. The percentages of apoptotic cells were 6.0%,18.5%,30.0%, 12.7% in experimental groups and resveratrol group respectively. The effect on PC-3 cells was enhanced with increasing amount of grape juice. The effect of low dose group was weaker than that of resveratrol group, but medium and high dose groups were stronger. Conclusion: The anti-prostate cancer effect of grape juice is not only related to resveratrol contained but also to synergism with other components, and its mechanism might be the induction of PC-3 cells apoptosis.
