Effect of combination of ACEI and ARB on left ventricle in pressure-overloaded hypertrophy rats
- VernacularTitle:血管紧张素转化酶抑制剂与血管紧张素受体拮抗剂合用对腹主动脉狭窄高血压大鼠心肌肥厚的影响
- Author:
Xuguo LIANG
;
Xiumei ZHANG
- Publication Type:Journal Article
- Keywords:
myocardial hypertrophy;
angiotensin-converting enzyme inhibitor;
angiotensin II receptor blocker;
cytokines;
matrix metalloproteinases;
apoptosis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect of the combination of ACEI and ARB on left ventricle in pressure-overloaded hypertrophy rats, and also the relationship between left ventricular hypertrophy (LVH) and cytokines, matrix metalloproteinases, apoptosis, and apoptosis genes. METHODS: IL-6, IL-10, MMP-2 and MMP-9 were evaluated by ELISA method. TNF-alpha was measured with radioimmunoassays. Flow cytometer was used to measure the apoptosis ratio and the expression of Fas, Bcl-2 and Bax. RESULTS: 6 weeks after abdominal aortic coarctation operation, the weight of the left ventricle ( 0.983 ? 0.316 g ) and heart weight/body weight ( 0.382 ? 0.154 ) were significantly increased in control group compared with the sham operated group. And also IL-6 ( 208.71 ? 84.51 pg?ml -1 ), TNF-? ( 1.983 ? 0.842 ng?ml -1 ), MMP-2 ( 74.63 ? 37.62 ), MMP-9 ( 57.74 ? 25.61 ), apoptosis ratio ( 8.47 ? 4.18 ) and apoptosis genes (fas, bax, bcl-2, bcl-2/bax) expression were increased, but IL-10 ( 86.23 ? 40.13 pg?ml -1 ) was decreased compared with sham operated group. The 3 groups with drugs (CP, LT, CP+LT) decreased the increasing weight of LV and ventricular weight/body weight and inhibited the changes of influencing factors (IL-6, IL-10, TNF-?, MMP-2, MMP-9, apoptosis ratio and apoptosis genes expression), especially in the group of combination of 2 drugs. CONCLUSION: LVH might be related with many influencing factors. ACEI and ARB may have the positive importance in the treatment and prevention of LVH.
