Influence of a self-designed multiple organ preservation solution on apoptosis of renal cortical cells during hypothermic storage
- VernacularTitle:SMO保存液对犬肾低温保存期间皮质细胞凋亡的影响
- Author:
Qiang NIU
;
Youhua ZHU
- Publication Type:Journal Article
- Keywords:
organ preservation;
reactive oxygen species;
hypothermia;
apoptosis
- From:
Academic Journal of Second Military Medical University
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the influence of a self-designed multiple organ preservation solution,namely Shanghai multi-organ preservation solution(SMO),on the apoptosis of canine renal cortical cells during cold storage and the related mechanism.Methods: Canine kidneys were freshly perfused and separately stored with cold(0-4℃) SMO,hypertonic adenine citrate solution(HC-A),and University of Wisconsin solution(UW solution)(the latter 2 as control).At defined time points during storage,the morphologic changes of renal cortical samples were observed;apoptosis of renal cortical cell was determined by TUNEL;changes of malonldialdehyde(MDA) in cortex homogenate were measured by thiobarbituric acid method;and the activity of superoxide dismutase(SOD) was determined by xanthine oxidase method.Results: Pathologic assessments indicated that the kidney damage was similar in 3 groups within 24 h.The kidney tubular cell swelling and necrosis in SMO group and UW group were slighter than those in HC-A group after stored for 2 days.With the prolongation of storage time,apoptosis increased in all groups,with the apoptotic index of SMO group significantly lower than that of HC-A group(P
