The research of drug serum on calcium and NOS-NO system in cardiomyocytes injured by hypoxia and reoxgyenization
- VernacularTitle:药物血清对缺氧/复氧心肌细胞Ca~(2+)及NOS-NO系统的相关性研究
- Author:
Xiao HAN
;
Jianxun LIU
- Publication Type:Journal Article
- Keywords:
serum pharmacology of chinese herbal medicine;
hypoxia and reoxygenation;
calcium;
nitric oxide synthase;
nitric oxide
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of shuang shen tong guan(SSTG) drug serum on calcium and NOS-NO system in cardiomyocytes injured by hypoxia and reoxgyenization.Methods The cardiomyocytes were deprived of oxygen and glucose to mimic hypoxia reoxygenation injury.Intracellular calcium concentration was determined by Fluorospectrophotometry.NOS activity and NO contents were detected using colorimetric method.Results Intracellular calcium concentration,NOS activity and NO content were increased after hypoxia and reoxygenation injury.SSTG drug serum inhibted the upwards of intracellular calcium concentration,activity of total NOS,indusive NOS and NO content due to hypoxia/reoxygenation injury significantly(P
