Effects of 1?,25-Dihydroxyvitamin D_3 on the proliferation and apoptosis of K562 leukemia cells and its mechanism
- VernacularTitle:1,25-二羟基维生素D_3抑制K562细胞增殖及其机制
- Author:
Yi ZHANG
- Publication Type:Journal Article
- Keywords:
1?,25-Dihydroxyvitamin D_3;
K562 cell line;
cell proliferation;
apoptosis;
Caspase-3
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
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Abstract:
Aim To investigate effects of 1?,25-Dihydroxyvitamin D_3 on the growth inhibition,proliferation of K562 cells and to explore its mechanism. Methods The expression of vitamin D receptor(VDR) was indentified by indirect immunofluorescent stainings.Cell growth,proliferation,apoptosis and cells cycle were evaluated by MTT assay,acridine orange/ethidium bromide(AO/EB) and flow cytometry PI staining.The enzymatic activity of the caspases-3 class of K562 cells was determined by colorimetric assay.Results ① VDR was present in K562 cell nucleus;② 10~(-8) mol?L~(-1) 1,25(OH)_2D_3 could markedly inhibit K562 cells growth and induce cells apoptosiswith most of cells being arrested in G_2/M phase.The ratio of apoptosis increased from 4.1%(control group) to 26.5%(treatment group),P
