Silymarin and agonistic Fas antibody,CH11,induced A375-S2 cell apoptosis through mitochondrial signal pathway
- VernacularTitle:水飞蓟素和Fas激动性抗体CH11共同作用诱导A375-S2细胞凋亡
- Author:
Xiaoke YANG
- Publication Type:Journal Article
- Keywords:
silymarin;
CH11;
synergism;
A375-S2 cell;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the synergism of silymarin and CH11 on A375-S2 cell apoptosis.Methods Inhibitory effects on A375-S2 cell lines were measured by crystal violet test.Photomicroscopical observation and LDH activity test were used to assess apoptosis.SIRT1,Bcl-2 and Bcl-xL were detected by western blot analysis.Results Treatment with silymarin and CH11 significantly induced apoptosis in A375-S2 cells in a time-dependent manner.A375-S2 cells incubated with silymarin and CH11 for 12 h showed marked apoptotic changes including condensed chromatin,nuclear fragmentation and apoptotic bodies.Expressions of anti-apoptotic Bcl-2 and Bcl-xL(Bcl-2 family member) were decreased simultaneously.Expressions of apoptotic Bax and cytochrom C were increased.Consequently,Caspase-3,downstream of mitochondria signal pathway, more significantly expressed after A375-S2 cells treatment with silymarin and CH11 for 12 h.Conclusion Silymarin and CH11 synergistically induced A375-S2 cells apoptosis.
