Effects of pioglitazone on cardiac fibroblast proliferation induced with high glucose and high insulin
- VernacularTitle:吡格列酮对高糖高胰岛素诱导的心肌成纤维细胞增殖的影响
- Author:
Keyan LIANG
;
Hongxin WANG
;
Pin BAO
- Publication Type:Journal Article
- Keywords:
pioglitazone;
high glucose;
high insulin;
cardiac fibroblast;
proliferation
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study effects of pioglitazone on cardiac fibroblast proliferation induced with high glucose and high insulin.Methods The neonatal rat cardiac fibroblasts were cultured and treated with different factors.The cellular number was determined by crystal violet uptake;the DNA synthesis was assayed with thymidine intake method;the percentage of S+G_2+M in the cell cycle was determined by flow cytometry.Results Pioglitazone inhibited the cell number、DNA synthesis and the percentage of S+G_2+M in the cell cycle of cultured cardiac fibroblasts induced with high glucose and high insulin.Conclusion Pioglitazone inhibits cardiac fibroblast proliferation induced with high glucose and high insulin and the effective mechanism of pioglitazone on cardiac fibroblast proliferation needs further study.
