Research of recombination phospholipase C
- VernacularTitle:重组磷脂酶C的研究
- Author:
Ning YANG
- Publication Type:Journal Article
- Keywords:
phospholipase C;
recombination PLC;
purification
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Phospholipase C(PLC) is a kind of hydrolase for phosphoinositide(PI),which plays a vital role in life activity as a second messenger.It can prohibit hematoblast from aggregating.PLC indwells the prokaryote and eukaryote,especially those existing in bacteria draw great attention since it can simulate the action in eukaryote.In this article,we describe the basic mechanism of cloning,refolding,expressing of PLC,and the purification of recombinant PLC. At the same time,we report our study briefly,and we wish the recombinant PLC will be developed into a new medicine of anti-aggregation to platelet.
