Expression of connective tissue growth factor and low density lipoprotein receptor related protein induced by transforming growth factor beta 1 in human pulmonary fibroblasts-1
- VernacularTitle:转化生长因子?_1对人胚肺成纤维细胞结缔组织生长因子及低密度脂蛋白受体相关蛋白表达的影响
- Author:
Yan WANG
;
Xinmin LIU
- Publication Type:Journal Article
- Keywords:
Transforming growth factor beta;
Connective tissue growth factor;
LDL-receptor related proteins;
Fibroblasts
- From:
Journal of Peking University(Health Sciences)
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the tendency and correlation between connective tissue growth factor (CTGF) and low density lipoprotein receptor-related protein (LRP) in human pulmonary fibroblasts-1 (HPF-1) induced by transforming growth factor beta1 (TGF-?_1) at different times. Methods: After the HPF-1 cells were stimulated with TGF-?_1 (5 ?g/L) at different times (0 h, 3 h,6 h,12 h and 24 h),CTGF and LRP mRNA expressions were analyzed by RT-PCR. The same preparation steps of cell culture were repeated, then, the protein expressions were determined by Western Blot, co-immunoprecipitation Western Blot and immunocytochemistry. Results: The expressions of CTGF and LRP mRNA had similar tendency, and LRP (224.87?7.00) correlated with CTGF (131.53?2.86) positively (r= 0.8402, P
