Influence of Retn gene expression on glucose uptake by 3T3-L1 cells and the related mechanism
- VernacularTitle:Retn基因表达对3T3-L1脂肪细胞葡萄糖摄取的影响及其机制探讨
- Author:
Yahui LI
- Publication Type:Journal Article
- Keywords:
resistin;
Retn gene;
insulin;
glucose uptake;
3T3-L1 cell line
- From:
Academic Journal of Second Military Medical University
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of high expression of Retn gene on glucose uptake by 3T3-L1 cells and to study its mechanism in inducing insulin resistance.Methods:(1)Radioimmunoassay was used to determine the glucose uptake by 3T3-L1 cells with low-,normal-and high-level Retn expression under basal and insulin-stimulated states.(2)RT-PCR and real-time RT-PCR were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different expression of Retn,including insulin receptor substrate-1(IRS-1),phosphatidylinositol 3-kinase(PI-3K),AKT-2,glucose transporter-4(GLUT-4),p38 mitogen-activated protein kinase(p38MAPK),and glycogen synthase kinase-3?(GSK-3?).(Results:) The uptake of glucose decreased with the increase of Retn expression under basal and insulin-stimulated conditions.The mRNA expression of 2 signal protein PI-3K and AKT-2 decreased with the increase of Retn expression;and the expression of GSK-3? and p38MAPK increased with the increase of Retn expression.Conclusion: Resistin protein can induce insulin resistance in adipocytes,which might be related to the expression changes of some proteins in PI-3K and Ras pathways.
