Micropatterning of endothelial cells on poly-dimethylsiloxane flow channel bottom
- VernacularTitle:流动腔底面上内皮细胞图型化方法的初步研究
- Author:
Li ZHANG
- Publication Type:Journal Article
- Keywords:
flow channel;
poly-dimethylsiloxane;
patterning;
micro-contact printing(?CP);
computational simulation
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To simulate the blood flow features of the susceptible sites of atherosclerosis in vitro.The cell micro-patterning is to be obtained on the bottom of channel for the information about single cell or cell groups.Methods Poly-dimethylsiloxane(PDMS) flow channel and PDMS stamp were fabricated by soft lithography.Fibronectin pattern was formed on chamber bottom by micro-contact printing method and cells selectively attached on fibronectin pattern.Results The PDMS-patterned chamber that possessed favorable optical property and the PDMS stamps with different sizes were obtained.Cells attached on fibronectin pattern presented square array.Conclusion The fabricated methods are quick and simple.The chamber could be used for the advanced real-time investigation of cell mechanical behavior in vitro.
