Protective effect of lipopolysaccharide preconditioning on ischemia/reperfusion injury of rat graft liver
- VernacularTitle:脂多糖预处理对大鼠肝移植再灌注期肝脏的保护作用及机制
- Author:
Yang LI
;
Zuojin LIU
;
Jianping GONG
- Publication Type:Journal Article
- Keywords:
liver transplantation;
reperfusion injury;
lipopolysaccharide
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect and the possible mechanism of lipopolysaccharide(LPS) preconditioning on ischemia/reperfusion injury of rat graft liver.Methods Male Sprague-Dawley rats were divided into three groups(n=30 in each group): sham operation group(Sham group),orthotopic liver transplantation group(OLT group) and LPS preconditioning group(LPS group).Only dissecting hepatoduodenal ligament was perfomed in Sham group.On day one,0.1 mg/kg LPS and on day 2,3,4,5,0.5 mg/kg LPS for LPS rats and 0.5 ml PBS pathogen-free solution for OLT rats was injected through caudal vein before OLT was performed by two-cuff method in the two group rats on day 8.The levels of tumor necrosis factor-?(TNF-?),ALT, AST in inferior caval vein blood and the activities of NF-?B in hepatic tissue were detected at 0,60,180 min after dissecting hepatoduodenal ligament in Sham group and after portal vein reperfusion in OLT group and LPS group.The morphological changes of hepatic tissue were observed through light microscope and electron microscope at different time points.Results As compared with Sham group at the different time points respectively,the activities of NF-?B and the levels of TNF-? were higher in OLT group and LPS group(P
