Effect of ?-crystallin on retinal ganglial cells
- VernacularTitle:?晶体蛋白对视网膜神经节细胞作用的初步研究
- Author:
Xiaoqin WANG
- Publication Type:Journal Article
- Keywords:
retinal ganglial cells;
?-crystallin;
immunofluorescence
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of ?-crystallin on retinal ganglial cells(RGCs) and the possible mechanisms.Methods The changes of RGCs in vitro stimulated by 10~(-7),10~(-6),10~(-5),or 10~(-4)g/L ?-crystallin for 1,3,12 h,1,2,3 d were observed and identified by Thy1.1 antibody.The number and the length of outgrowing RGCs axons were calculated under microscope and compared with the control group.The effect of ?-crystallin on RGCs was detected by immunofluorescence with confocal laser scanning microscope.Results The length and number of RGCs axons in 10~(-4),10~(-5)g/L ?-crystallin solution were more than in the control on day 1,2,3 after ?-crystallin stimulation,most significant difference in 10~(-4)g/L group.The interaction between ?-crystallin and RGCs was proven positive by immunofluorescence with confocal laser scanning microscope.It increased firstly,then reduced.It reached the maximum at 3 h after ?-crystallin stimulation.Conclusion ?-crystallin enhanced the survival of RGCs and the regeneration of axons,suggesting ?-crystallin might be related to the changes of RGCs membrane signal transduction pathway.The mechanism might be involved in the interaction between ?-crystallin and RGCs.
