Effect of different cryopreservation time on energy metabolism in rat liver transplantation
- VernacularTitle:不同冷保存时间对大鼠移植肝脏能量代谢的影响
- Author:
Ying ZHANG
;
Ping BIE
;
Qiao WU
;
Kun LI
- Publication Type:Journal Article
- Keywords:
liver transplantation;
ischemia/reperfusion injury;
rat
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different cryopreservation time on energy metabolism and reperfusion injury in rat to rat liver transplantation and the mechanism of liver function recovery in early period after operation.Methods The cryopreservation time of donor livers from 90 Wistar rats were 30 min,6 or 12 h in 4 ℃ UW solution.Then orthotopic liver transplantation were performed in another 90 Wistar rats.Another 6 rats served as normal controls.At 12 h,24 h,3 d,5 d,7 d after portal vein reperfusion,blood and liver tissue were obtained to determine the levels of ALT,AST,SOD,ATPase and ATP,and the pathologic changes of liver tissue were observed.Results At 12 h after operation,the levels of ALT and AST were significantly higher(P
