Antisense oligodeoxyribonucleotide of pituitary tumor transforming gene inhibits proliferation of glioblastoma
- VernacularTitle:垂体瘤转化基因反义寡核苷酸抑制胶质瘤增殖的实验研究
- Author:
Yingxin CHENG
;
Yuan GAO
;
Wenyuan TANG
;
Bingbo CHEN
- Publication Type:Journal Article
- Keywords:
glioblastoma;
pituitary tumor transforming gene;
ASODN
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibition effect of pituitary tumor transforming gene(PTTG) antisense oligodeoxynucleotide(ASODN) on C6 glioblastoma in rats.Methods The C6 glioma cells were injected into the right caudate nucleus.PTTG-ASODN of 8 or 16 ?g/ml was injected into the tumor-affected area with stereotactic technique immediately,at 1st and 2nd week after inoculation of C6 cells.Three weeks after C6 cell inoculation,all rats were killed and the tumors were excised,then tumor volume was calculated and pathologically analysed,and immunohistochemical statining for GFAP, PCNA ang PTTG was performed.Results PTTG-ASODN could suppress the proliferation of C6 glioblastoma in a dose-and time-dependent manner.The inhibition effect was better when large-dose PTTG-ASODN was repeatedly used for glioblastoma as early as possible.Conclusion PTTG-ASODN can suppress the proliferation of glioblastoma,which may become a new strategy of gene therapy for glioblastoma.
