ERK1/2 pathway play a important role in the apoptosis of glomerular mesangial cells induced by TIMP-1
- VernacularTitle:ERK1/2通路在TIMP-1抑制大鼠肾小球系膜细胞凋亡中的作用
- Author:
Yanling SUN
;
Hongli LIN
;
Changqing YU
- Publication Type:Journal Article
- Keywords:
ERK1/2;
TIMP-1;
Rat mesangial cell apoptosis
- From:
Chinese Journal of Practical Internal Medicine
2006;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of ERK1/2 signal pathway in the apoptosis of rat mesangial cells inhibited by TIMP-1.Methods The human TIMP-1 sense and antisense plasmid were transferred into rat mesangial cells through liposome,while mesangial cells transfected with pcDNA3 and non-transfected mesangial cells were used as control.After being stimulated with high glucose(HG 25mmol/L D-glucose)and PD98059(50 ?mol/L)for 24 hours,the apoptosis rate was measured by flow cytometry.The expression of ERK1mRNA was assayed by RT-PCR.The protein expression of p-ERK1/2 in the cell was analyed by ELISA.Results(1)The apoptosis rates of the non-transfected group,transfect pcDNA3 group,sense group and antisense group in the culture medium of high glucose withact PD98059,were(12.10?2.21)%,(11.90?3.34)%,(5.50?0.50)%and(20.70?3.41)%,respectively.The apoptosis rate of the sense group was significantly lower than that without transfection(P
