Detection of Plasmodium sporozoites in mosquitoes by nested-PCR
- VernacularTitle:套式PCR检测蚊体内疟原虫的研究
- Author:
Huayun ZHOU
;
Guoding ZHU
;
Xiaolin JIN
;
Julin LI
;
Weiming WANG
;
Jun CAO
;
Yaping GU
;
Qi GAO
- Publication Type:Journal Article
- Keywords:
PCR;
Anopheles anthropophagus;
Anopheles dirus;
Anopheles sinensis;
Plasmodium
- From:
Chinese Journal of Schistosomiasis Control
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a fast and sensitive method to detect Plasmodium sporozoites in mosquitoes. Methods Anopheles anthropophagus (An.a) artificially infected with Plasmodium vivax (P.v), Anopheles dirus (An.d) infected with Plasmodium falciparum (P.f) and both of P.v and P.f, field mosquitoes of Anopheles sinensis (An.s) captured in epidemic seasons were detected by nested-PCR. Results The results of 28 batches of An.a infected with P.v, 2 batches of An.d with P.f and 1 batch of An.d mixed with P.v and P.f by nested-PCR were accorded with the microscopical examination absolutely. Two positives of 589 An.s field mosquitoes were discovered with a positive rate of 0.34%. Conclusion The nested-PCR is fast and sensitive for detecting different species of Plasmodium in mosquitoes.
