Studies on the function of FBG2 in gastric cancer cell lines
- VernacularTitle:泛素代谢系统相关基因FBG2在胃癌细胞系中功能意义的研究
- Author:
Lin ZHANG
;
Mengwei WANG
;
Benyan WU
- Publication Type:Journal Article
- Keywords:
stomach neoplasms;
genes, FBG2;
cell cycle;
transfection
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of FBG2 on the growth, proliferation, apoptosis, infiltration and cell cycle of the gastric cancer line MKN45. Methods A critical component ubiquitin-protein ligase complex, FBG2 cDNA, was subcloned into a constitutive vector pcDNA3.1, followed by transfection in MKN45 by using liposome. Then stable expression clones were selected and appraised. The apoptosis and cell cycles were detected using flow cytometry. The growth and proliferation were analyzed by plotting cell growth curves and colony formation assay respectively. The ability of infiltration was tested using cancer cell migration assay. The MKN-FBG2 group and two control groups were included in the study. Results MKN-FBG2 grew faster than MKN45 and MKN-PC. The cell counts of MKN-FBG2 on the fourth, fifth, sixth and seventh day were significantly larger than that of others (P
