Long serial analysis of gene expression in the yeast phase of Candida albicans
- VernacularTitle:白念珠菌酵母相细胞长片段基因表达系列分析文库的构建和初步分析
- Author:
Cunjian ZHOU
;
Fei HAO
;
Yongjian DENG
- Publication Type:Journal Article
- Keywords:
Candida albicans;
dimorphism;
gene expression profiling;
long serial analysis of gene expression
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a library of long serial analysis of gene expression (LongSAGE) and investigate the characteristics of gene expression in the yeast phase of C. albicans. Methods A LongSAGE library was constructed with long serial analysis of gene expression (LongSAGE). Results A total of 17 773 tags, representing specific 7 333 transcripts, were extracted from 1 000 sequence files in the yeast phase of C. albicans. Tags matching single gene accounted for 16.65%, tags matching multiple genes accounted for 6.59%, tags for hypothetical protein accounted for 16.27%, tags for genome and cosmid accounted for 1.64%, and novel tags accounted for 58.86%. Eleven tags in 33 tags whose expression frequencies exceeded 35 may be new expression sequences. Conclusion LongSAGE is not only a powerful method in investigating the gene expression profiling, allowing the analysis of the expression of thousands of transcripts in a quantitative manner without prior sequence information, but also in finding new genes.
