Relationship between the differently expressed gene and pulmonary thromboembolism
- VernacularTitle:mRNA差异表达片段与肺血栓栓塞的相关性研究
- Author:
Yun GU
;
Ying LIANG
;
Jinghua YANG
- Publication Type:Journal Article
- Keywords:
Pulmonary thromboembolism;
Gene expression;
Peripheral blood mononuclear cells
- From:
Chinese Journal of Practical Internal Medicine
2006;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To search for and investigate the relationship between the differently expressed gene and pulmonary thromboembolism.Methods Peripheral blood mononuclear cells were isolated from pulmonary thromboembolism patients who were diagnosed and sex,age matched healthy controls.To screen differently expressed mRNA fragment which was related to pulmonary thromboembolism.Analyze one of the new ESTs.Simultaneity and homology comparison were made.Results First,fifty-five candidate mRNA fragments were chosen.Analysis of ten candidate mRNA fragments among them(up regulation or down regulation more than 3 times)were made by using Homology search,which confirmed that g44 and g48 were new sequence and were registered to GenBank.Making g44 be probe,northern blot was used to analyze one of the new ESTs.Signal of cross was found in controls and patients with pulmonary thromboembolism.It was significantly strong in the patient group compared with the control group.Conclusion These results reveal that new fragments have a strong expression in pulmonary thromboembolism group than control group.It suggests that these two new gene fragments may play an important role in the process of pulmonary thromoembolism as pathogenic gene.
