Experimental Research on Primary Culture of Improved Rats’ Osteoblast
- VernacularTitle:改良大鼠成骨细胞原代培养方法的实验研究
- Author:
Chengliang WU
;
Luwei XIAO
;
Peijian TONG
- Publication Type:Journal Article
- Keywords:
osteoblast;
primary culture;
rats;
experimental research
- From:
Journal of Zhejiang Chinese Medical University
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Get a primary culture method of osteoblast which is simple to operate and has many pure cells. Method Get osteoblast with improved I collagenase, and test the form of osteoblast, the alkaline phosphatase dyeing and formation of mineralization node. Result The improved rats primary culture method of osteoblast can get most cells in normal shape, alkaline phosphatase secretion and mineralization. Conclusion The improved rats primary culture method of osteoblast is convenient to operate, and can get most cells.
