Activators of peroxisome proliferator-activated receptors inhibit cultured AngⅡ-induced hypertrophic cardiomyocyte from neonatal rats
- VernacularTitle:过氧化物酶体增殖激素型受体对AngⅡ诱导肥厚心肌细胞的影响
- Author:
Yongyao YANG
- Publication Type:Journal Article
- Keywords:
peroxisome proliferator-activated receptors;
fenofibrate;
pioglitazone;
cardiac hypertrophy
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of the ligands of peroxisome proliferator-activated receptors(PPARs),fenofibrate(PPAR? activator) and pioglitazone(PPAR? activator)on the angiotensin Ⅱ(Ang Ⅱ)-induced cardiac hypertrophy in vitro.Methods A model of hypertrophy of neonatal rat cardiac myocytes was established with AngII stimulation.With the aid of Leca Qwin Image software,the surface area of cardiac myocytes was analyzed.The mRNA expression of ?-MHC,?-MHC was measured by reverse transcription-polymerase chain reaction(RTPCR) and the cultured myocyte viability was estimated by MTT assay.Results Fenofibrate or pioglitazone pretreatment 24 h prior to AngⅡ stimulation,significantly reduced the cardiac hypertrophy(P
